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 gel electrophoresis 添加此单词到默认生词本
【化】 凝胶电泳




    electrophoresis \electrophoresis\ n.
    1. (Chem.) the motion of charged molecules or particles in a
    liquid medium under the influence of an electric field;
    particles with a positive charge move toward the cathode
    and negative to the anode. [WordNet sense 1]

    Syn: cataphoresis.
    [WordNet 1.5]

    2. (Chem., Biochem.) the application of the principle of
    electrophoresis to separate molecules, used as an
    analytical or preparative technique; as, separation by
    electrophoresis; gel electrophoresis.
    [PJC]

    Note:

    {Gel electrophoresis} is a technique in which the molecules
    to be separated are moved through a gelatinous medium
    under the influence of an electric field. At the
    completion of a period of electrophoresis, the gel, unlike
    a liquid solution, may be manipulated as a single object,
    permitting the substances contained within to be detected
    or visualized by a variety of methods, and their relative
    mobilities determined. It is therefore a popular analytic
    tool in biochemistry, and has many variants. Popular
    substances used to create the gel are starch,
    polyacrylamide, and agarose. Since a polyacrylamide gel
    can be created with different concentrations and different
    degrees of cross-linking, the pore size of the gel can be
    controlled to provide special properties appropriate to
    separation of specific molecules, as for example
    optimizaion for separation within a particular molecular
    weight range. in SDS-polyacrylamide gel electrophoresis,
    SDS ({sodium dodecyl sulfate}, a detergent) is included;
    it binds to and denatures protein molecules, allowing them
    to be separated on the basis of their molecular weight
    alone. It is thus used as one method of determining the
    molecular weights of isolated protein chains.
    [PJC]

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