带出物 [
dài chū wù]
carry-over
- 从131个引物中筛选出5个重复性好、多态性高的引物,共扩增出302条谱带,其中111条谱带具有遗传多态性.
Five primers with good repetition and high polymorphism were screened from 131 primers,and a total of 302 bands were amplified,among which,111 bands were of genetic polymorphism. - 最后,以27个甜樱桃种质的基因组DNA为模板,从27对可扩增出带的引物中,筛选出多态性引物24对,获得了24个甜樱桃基因座特异性SSR标记。
24 polymorphic primer pairs were selected from the 27 primer pairs by using the genomic DNA of 27 sweet cherry germplasm,and 24 locus-specific SSR markers were obtained. - 两引物均能在父母本中扩出特异带,为共显性标一记,不仅能够鉴别杂交种中混杂的母本自交的种子和父本的种子,还可鉴别部分由于外来花粉或机械混杂等造成的假杂种。
Both of them, to be codominant markers, can identify false hybrids not only from selfed seeds of male and female but also from foreign pollen and mechnical contamination.
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